E9770
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 | Transfection of Sf9 cells with baculovirus DNA containing a GFP (Green Fluorescent Protein) reporter gene. Transfection procedure was performed according to the recommended protocol for each reagent (ESCORT, Competitor A, Competitor B). 1×106 Sf9 insect cells were plated in 3.5 cm well of a 6 well plate containing serum free insect cell culture medium and transfected using different amounts of liposome transfection reagent and DNA (see table). Higher and brighter fluorescence is seen using ESCORT indicating higher expression of GFP. |
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E9770
Sigma
Escort™ Transfection Reagent
2 mg/mL, Molecular Biology
| MDL number: | MFCD00676963 |
Description
| Application | Efficient transfection has been demonstrated in a wide variety of cell lines including: HeLa cells, CHO-K1, COS 7, CV 1, HEK 293, PAE and Sf9 insect cells. In particular, ESCORT is the transfection reagent of choice for Sf9 insect cells resulting in higher efficiencies, better cell viability and requiring less transfection reagent and less DNA compared to other insect specific transfection reagents. |
| Features and Benefits | • Efficiently transfects wide variety of cell types • Low toxicity • Suitable for stable and transient transfection • Use in full-serum or serum-free media • Economical • Ideal for Sf9 insect cells • Less DNA required • High cell viability after transfection |
| Other Notes | ESCORT liposome transfection reagent is a highly effective, low toxicity transfection reagent suitable for both transient and stable transfection in either full serum or serum free medium. It is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTAP) and dioleoyl phosphatidylethanolamine (DOPE) in 0.2 μm filtered MES-buffered saline, pH 6.2. |
| Quantity | Using HeLa cells with the pSV40-CAT reporter gene, 5 μl is sufficient to transfect a 35 mm culture dish; 15 μl is sufficient for a 60 mm dish. The optimal volume required for transfection may vary from cell line to cell line. |
| Legal Information | Escort is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Molecular Biology |
| concentration | 2 mg/mL |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 3 |
